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https://cris.library.msu.ac.zw//handle/11408/1538
Title: | HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions | Authors: | Moyo, Mambo Jonathan, Okonkwo O. Nana, Agyei M. |
Keywords: | Horseradish peroxidase, Maize tassel, Multi walled carbon nanotubes, Amperometric biosensor, Healy metal, Inhibition | Issue Date: | 2013 | Publisher: | IEEE | Series/Report no.: | Sensors;p. 1 - 4 | Abstract: | The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. Using Cd2002B; as a model divalent metal ion, the inhibition rate was proportional to the concentration in the range from 0.002-0.030 mg L-1 with a limit of detection of 0.51 μg L-1. Representative Dixon and Cornish-Bowden plots showed that the reaction was reversible and noncompetitive. | URI: | http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6688375 http://hdl.handle.net/11408/1538 |
ISSN: | 1930-0395 |
Appears in Collections: | Research Papers |
Files in This Item:
File | Description | Size | Format | |
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HRP biosensor based on carbonized maize tassel.pdf | Abstract | 181.81 kB | Adobe PDF | View/Open |
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