Please use this identifier to cite or link to this item: https://cris.library.msu.ac.zw//handle/11408/1538
Title: HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions
Authors: Moyo, Mambo
Jonathan, Okonkwo O.
Nana, Agyei M.
Keywords: Horseradish peroxidase, Maize tassel, Multi walled carbon nanotubes, Amperometric biosensor, Healy metal, Inhibition
Issue Date: 2013
Publisher: IEEE
Series/Report no.: Sensors;p. 1 - 4
Abstract: The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. Using Cd2002B; as a model divalent metal ion, the inhibition rate was proportional to the concentration in the range from 0.002-0.030 mg L-1 with a limit of detection of 0.51 μg L-1. Representative Dixon and Cornish-Bowden plots showed that the reaction was reversible and noncompetitive.
URI: http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6688375
http://hdl.handle.net/11408/1538
ISSN: 1930-0395
Appears in Collections:Research Papers

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